Quantitative vitellogenin (Vtg) ELISA kits for detection of endocrine disruptors with estrogenic or anti-estrogenic properties.
Vtg is one of the core endpoint in screening and testing for endocrine disrupting chemicals (EDCs) described in the OECD Guidelines for the testing of chemicals, for example:
OECD Test guideline 229 (Fish Short-Term Reproduction Assay)
OECD Test guideline 230 (21-day Fish Assay)
OECD Test guideline 234 (Fish Sexual Development Test)
OECD Test guideline 240 (MEOGRT)
The Biosense vitellogenin ELISA kits have been developed for the standard fish test species recommended by OECD, and are used in many ecotoxicology laboratories throughout the world. The kits come in two sizes, with 96 and 480 wells (1 and 5 plates), and contains all necessary reagents for running the ELISA as well as an instructive protocol.
V01018401-096: Fathead minnow (Pimephales promelas) vitellogenin ELISA kit, 96 wells
V01018401-480: Fathead minnow (Pimephales promelas) vitellogenin ELISA kit, 480 wells
User Guide
V01008402-096: Zebrafish (Danio rerio) vitellogenin ELISA kit, 96 wells
V01008402-480: Zebrafish (Danio rerio) vitellogenin ELISA kit, 480 wells
User Guide
V01013403-096: Japanese medaka (Oryzias latipes) vitellogenin ELISA kit, 96 wells
V01013403-480: Japanese medaka (Oryzias latipes) vitellogenin ELISA kit, 480 wells
User Guide
V01004402-096: Rainbow Trout (Oncorhynchus mykiss) vitellogenin ELISA kit, 96 wells
V01004402-480: Rainbow Trout (Oncorhynchus mykiss) vitellogenin ELISA kit, 480 wells
User Guide
V01002402-096: Semi-quantiative vitellogenin Salmonid (Salmoniformes) biomarker ELISA kit, 96 wells
V01002402-480: Semi-quantiative vitellogenin Salmonid (Salmoniformes) biomarker ELISA kit, 480 wells
User Guide
V01006401-096: Cod (Gadus morhua) Vitellogenin ELISA kit, 96 wells
V01006401-480: Cod (Gadus morhua) Vitellogenin ELISA kit, 480 wells
User Guide
New - Mab/Mab ZF Vtg kit available
Compared to our polyclonal zebrafish Vtg assay (V01008402) our new Mab-Mab zebrafish Vtg assay (V01008403) offer some advantageous features: Ready to use TMB substrate over OPD tablet substrate, shorter incubation time (2.5 hour over 3.5 hour for the polyclonal). Also with the new Mab/Mab kit minimum sample dilution needed to avoid matrix effect is 1:50 for Whole Body Homogenate samples, compared to 1:500 for the polyclonal kit. Lower minimum sample dilution can improve the overall sensitivity for WBH samples.
V01008403-096: Mab/Mab Zebrafish(Danio rerio) vitellogenin ELISA kit, 96 wells
V01008403-480: Mab/Mab Zebrafish(Danio rerio) vitellogenin ELISA kit, 480 wells
This guide is made to help users to troubleshoot issues that might occur when running ELISA assays. The guide is based on feed-back from users and in-house experience with our Vtg assays and has a “issue - possible cause & solution” set-up.
The guide focus on the Vtg ELISA assay. If you have questions regarding sampling & sample preparation of plasma and WBH, general experimental set-up, variation between individual specimens etc, please contact us and we will do our best to help.
We also offer a variety of vitellogenin antibodies and standards. Please click here for more info.
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